Laboratory Applications in Microbiology Information Center: What's New


		Table of Contents About the Author Book Preface Feature Summary What's New McGraw-Hill Create



	Student Edition
	Instructor Edition

Laboratory Applications in Microbiology: A Case Study Approach, 3/e

Barry Chess, Pasadena City College

ISBN: 0073402427
Copyright year: 2015

What's New

Changes to the Third Edition

When Apple released its latest iPhone, it rolled out an advertising campaign focused on the fact that the new phone was both bigger (in terms of screen size) and smaller (at least thinner) at the same time. The subtext, of course, was that the phone had more of what people wanted, less of what they didn’t, and was easier to use, all at the same time. In that same spirit, the third edition of Laboratory Applications in Microbiology has incorporated a number of new features designed to keep the manual contemporary, make it easier to use, and improve the experience of both students and instructors. These changes include:

• New feature. “There’s More to the Story . . . ” serves as a jumping off point for students who want to go the extra mile. Broadly written, these ten addendums ask students to take the exercise they’ve just completed to the next step. After Exercise 17 (Lethal Effects of Ultraviolet Light) for example, students are encouraged to study the effects of environmental UV radiation on bacterial populations. Subsequent to an exercise on algae, students have a chance to receive training and become volunteer researchers for the Phytoplankton Monitoring Network. Following an exercise on milk spoilage (MBRT, ex 30), students can make—and study—their own fermented food. Whether used as extra credit, individual exercises, or even independent study projects, There’s More to the Story . . . requires students to do research, generate a protocol, and prove or disprove a hypothesis, in other words, act like the scientists we are training them to be.

• New organization. The case study for each exercise has been consolidated and moved to the end of the exercise. Exercises have also been reformatted to speed things along and make the case study optional. The laboratory procedure now immediately follows the introduction, allowing students and instructors to jump right in to lab work. Questions too have been rearranged and reformatted so that lower-order questions (knowledge, comprehension) appear first, with higher-level questions (analysis) following the lab exercise. The case study follows the lab and is followed in turn by several highest-order questions (synthesis and evaluation) based specifically on the events detailed in the study.

• New icon. Throughout the manual you will see a camera icon like this directing you to a specific page in the photo atlas. On that page you will find photos of organisms, cultural characteristics, biochemical or physiological results that will prove helpful.

• New case studies. Some interesting things have happened in the last few years, and you’ll find a dozen new case studies this time around. Exercise 1 on laboratory safety for instance now follows a nationwide outbreak of Salmonella that began in a microbiology teaching lab. It literally cannot get any more relevant. Additionally, cases throughout the manual have been updated to include any new information.

• New student learning outcomes (SLOs) have been added to each exercise. Found as part of the case study exercises in the second edition, expanding SLOs to the entire manual allows concrete evaluation of the skills and theory a student should master as they complete each exercise. The SLOs also allow instructors to easily track the skills they teach and correlate these to department and campus objectives.

• New photographs. Over three dozen new photographs can be found throughout the manual. Chosen because they display new techniques, alternative methods, or simply because each was better than the photograph it replaced, every new photo was chosen with only one thought in mind, how to make microbiology more understandable to the student.

A more comprehensive list of changes to the third edition includes the following:

Exercise 1: Laboratory Safety
• A new case study focusing on a nationwide Salmonella epidemic that had its start in a microbiology teaching laboratory.
• Updates to a UCLA lab fire, including the legal consequences for faculty.

Exercise 3: Identification and Classification of Algae
• New case study on toxic algae.
• There’s More to the Story . . . Algae monitoring as part of the Phytoplankton Monitoring Network.

Exercise 4: Medically Important Protozoa
• New case study on Naegleria fowleri infections connected to sinus rinsing.

Exercise 5: Identification and Classification of Fungi
• New case study on Histoplasma infections connected to a summer day camp.
• New information concerning fungal pathogenesis.

Exercise 6: Ubiquity of Microorganisms
• Three new case studies on hospital design and infection control.

Exercise 7: Aseptic and Pure Culture Techniques
• Pure culture and aseptic techniques have been combined into a single exercise.

Exercises 8–11: All staining exercises have been supplemented by including information previously found only in the laboratory techniques section of the manual.

Exercise 10: Endospore Staining
• A single exercise is completely devoted to endospore staining.
• There’s More to the Story . . . Isolation and identification of endospore-forming bacteria.

Exercise 11: Acid-Fast Staining
• A single exercise is completely devoted to acid-fast staining.
• New case study on Mycobacterium chelonae infections connected to tattoos.

Exercise 12: Viable Plate Count
• New case study concerning E. coli O157:H7 infections associated with raw milk.
• Additional information on the importance of coliforms.
• More background on viable plate counts along with tips to improve the success and reproducibility of the exercise.

Exercise 16: Effect of Osmotic Pressure on Bacterial Growth
• There’s More to the Story . . . Isolation of Halotolerant Microbes.

Exercise 17: Effects of Ultraviolet Light
• There’s More to the Story . . . Environmental effects of ultraviolet light.

Exercise 18: Evaluation of Disinfectants
• New case study concerning skin infections traced to wrestling equipment.
• There’s More to the Story . . . Disk Diffusion Testing of Disinfectants.

Exercise 19: Hand Scrubbing
• There’s More to the Story . . . Analysis of Skin Microbiota.

Exercise 20: Antimicrobic Susceptibility Testing
• There’s More to the Story . . . Isolation of Antibiotic-Producing Bacteria.

Exercise 24: Bacterial Transformation
• New case study, Hospital Outbreak of Carbepenem-Resistant Enterobacteriaceae.

Exercise 26: DNA Extraction from Bacterial Cells
• New case study, Ikea Pulls Meatballs from Store Shelves after Horsemeat DNA Detected.

Exercise 29: Membrane Filtration
• There’s More to the Story . . . Identification of Bacteria in Water.

Exercise 30: Methylene Blue Reductase Test
• There’s More to the Story . . . Production and Study of Yogurt.

Exercise 33: Isolation and Identification of Streptococci
• New case study, Streptococcus pneumoniae Outbreak in Nursing Facility.
• There’s More to the Story . . . Tracking Hand Hygiene

Exercise 34: Differentiation of Enterobacteriaceae
• E. coli O157:H7 outbreak tied to a Petting Zoo.

Instructors: To experience this product firsthand, contact your McGraw-Hill Education Learning Technology Specialist.