Biology (Raven), 10th Edition

Chapter 17: Biotechnology

Pre-Test

1
What is the purpose of cutting DNA resulting in staggered cuts that produce "sticky ends"?
A)These sticky ends allow DNAs from different sources to be easily joined together.
B)The sticky ends allow the cut fragments to anneal to one another.
C)ATP can hydrolyze the stop codons on sticky ends.
D)In order to produce vectors with full gene products, sticky ends must first be cut into them.
E)Creating sticky ends allows researchers to separate DNA fragments based on the nucleotide sequence of these ends.
2
What technique takes advantage of the charge on molecules by using an electrical field to provide the force necessary to separate the molecules based on size?
A)PCR
B)Hybridization
C)Southern Blot
D)Northern Blot
E)Electrophoresis
3
Why is Taq polymerase used in PCR rather than other DNA polymerases?
A)Taq polymerase is a synthetic enzyme that produces DNA strands at a faster rate than natural polymerases.
B)Taq polymerase is a heat-stable form of DNA polymerase that can function after exposure to the high temperatures that are necessary for PCR.
C)Taq polymerase is easier to isolate than other DNA polymerases.
D)Taq polymerase is the DNA polymerase commonly produced by most eukaryotic cells.
E)All of the above are correct.
4
The method of determining the base sequence of DNA is
A)PCR.
B)gene cloning.
C)DNA fingerprinting.
D)DNA sequencing.
E)gene mapping.
5
The most flexible and common host used for molecular cloning is a
A)protein.
B)plasmid.
C)virus.
D)yeast.
E)bacterium.
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