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1
The experimental insertion of a fragment of frog DNA into a bacterial plasmid produces a(n) ________ molecule; if this fragment contains a gene, the process is most accurately referred to as ________.
A)recombinant; gene cloning
B)diploid; splicing
C)hybrid; fusion
D)eukaryo-bacterial; genetic engineering
E)mutant; biosynthesis
2
Large sections of DNA cannot be introduced into plasmid or viral vectors due to
A)their inability to maintain structural integrity.
B)inability to produce polymerases capable of binding to the introduced DNA.
C)their inability to replicate large portions of DNA, causing deletions.
D)their inability to translate to large portions of DNA.
E)their inability to manufacture the protein products of large DNA segments.
3
Mapping of a complete chromosome requires many overlapping segments of DNA to be cloned within
A)BACs.
B)YACs.
C)viral vectors.
D)contigs.
E)prokaryotes.
4
A vector molecule is:
A)a fragment of DNA of interest, perhaps a gene to express or study.
B)another name for a bacterial chromosome.
C)often a plasmid, or occasionally a modified viral particle.
D)a naturally occurring molecule in the cytoplasm of an animal cell.
E)a protein used in the formation of recombinant DNA.
5
Restriction enzymes make useful vector molecules due to their ability to
A)to digest DNA at very specific and predictable locations.
B)degrade foreign DNA molecules.
C)be easily recognized.
D)their ability to produce sticky ends.
E)Restriction enzymes do not make useful vector molecules.
6
You are attempting to clone a gene of interest into the lacZ gene of a plasmid and introduce your recombinant molecule into a bacterial host sensitive (i.e. not resistant) to ampicillin. You grow your potential transformants on media containing both ampicillin and X-Gal. Which of the following results would most likely indicate a successful clone?
A)Cells which do not grow on the ampicillin plates
B)Cells which form white colonies on the ampicillin plates
C)Cells which form blue colonies on the ampicillin plates
D)Cells which form light blue (half blue, half white) colonies on the ampicillin plates
E)None of these results would indicate a successful clone.
7
A collection of recombinant vectors that represent the proteins expressed in a particular cell at a particular time is most accurately referred to as a
A)genomic library.
B)proteomic library.
C)colony.
D)cDNA library.
E)ds-DNA library.
8
True or False: Polymerase chain reaction (PCR) is a quantitative measurement of cloned DNA.
A)True
B)False
9
In gel electrophoresis
A)smaller DNA fragments move faster than larger DNA fragments.
B)DNA migrates towards a positive electrical pole.
C)smaller DNA fragments have more freedom of movement than larger fragments.
D)samples of DNA are loaded near the negative electrode of a gel and migrate towards the positive electrode.
E)All of these statements about gel electrophoresis are correct.
10
The polymerase chain reaction (PCR) is comprised of repeated cycles of the following steps
I: primer extension
II: annealing
III: denaturation
A)iii; i; ii
B)i; iii; ii
C)ii; i; iii
D)iii; ii; i
E)ii; iii; i
11
True or False: A dideoxynucleoside triphosphate molecule cannot be incorporated into a growing DNA chain because it lacks an -OH group at the 3' carbon.
A)True
B)False
12
Which of the following is not a ddNTP?.
A)ddATP
B)ddTTP
C)ddCTP
D)ddGTP
E)All of the choices are forms of ddNTPs.
13
Which of the following statements about DNA microarrays is correct?
A)Short regions of double stranded DNA are arranged on each gene chip.
B)RNA molecules are directly hybridized to the DNA on the microarray.
C)Microarrays are commonly used to study gene expression patterns.
D)Each microarray has multiple copies of the DNA, but only a single unique sequence per microarray.
E)The detectable marker used in DNA microarrays is typically radioactive phosphorous, since DNA has phosphate groups.
14
Many pharmaceutical products are engineered from livestock using
A)genetic mapping.
B)the chain termination method.
C)proteometric analysis.
D)molecular pharming.
E)transgenic proteomics.
15
Which of the following statements regarding transgenic plants is incorrect?
A)Many plant cells are totipotent, making it easier to produce transgenic plants.
B)Agrobacterium tumafaciens is most often used to transfer genes to plants using the T1 plasmid
C)Genes are placed in the T DNA region of the plant cells
D)Expressed T DNA results in a tumor called a crown gall tumor.
E)All of these statements regarding transgenic plants are incorrect.
16
True or False: When a cloned gene recombines with a normal gene on a chromosome, this is most accurately referred to as gene replacement.
A)True
B)False
17
Which one of the following statements about molecular pharming is incorrect?
A)The ultimate goal is to use farm animals to produce pharmaceuticals.
B)The strategy is to clone a gene near a promoter for a gene that is specifically expressed in mammary cells.
C)This approach has resulted in treatments for emphysema and hemophilia.
D)The yield of recombinant protein in milk can be 1 gram of protein per liter.
E)All of these statements about molecular pharming are correct.
18
You are trying to determine which pancreatic cells in a sheep pancreas are responsible for the production of insulin. Which of the techniques would be most appropriate to utilize?
A)DNA fingerprinting
B)Transgenic mice
C)Knockout mice
D)DNA microarray
E)Genetically modified organisms
19
Sites in the genome of a species which are commonly used for DNA fingerprinting are most accurately referred to as
A)contigs.
B)microarrays.
C)restriction sites.
D)short tandem repeats.
E)transgenes.
20
True or False: The first inherited disease treated with gene therapy was adenosine deaminase deficiency.
A)True
B)False





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