a. A mouse is irradiated so that its bone marrow and thymus are destroyed. It then is resupplied only with bone marrow. The mouse is unable to form antibodies. Why?
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b. A mixture of B cells is exposed to a specific radiolabeled antigen in vitro (within laboratory glassware). Would you expect all B cells to bind with the antigen and to be radiolabeled?
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c. When B cells and T cells are incubated in vitro with a radiolabeled antigen, binding to certain B cells occurs but not to T cells. Why?
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d. Human beings communicate by sight, sound, and touch. How do immune cells communicate with one another?
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2. In this text, antigen originally was defined as a foreign substance in the body.
a. Expand this definition by telling what an antigen does in the body.
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b. It is possible to tag different types of monoclonal antibodies with different dyes so you can tell them apart. Knowing this, how would you produce and use monoclonal antibodies to distinguish helper T cells and cytotoxic T cells in a blood sample?
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c. How would you prove that a monoclonal antibody is specific to the herpes virus (HSV-2) that causes genital herpes, but not to the one (HSV-1) that causes cold sores?
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d. In a manufacturing process called affinity purification, a mixture that contains a desired substance is passed through a tube. In the tube, a large number of antibody molecules are fixed to a solid support. Why will this process result in purification of the product?
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