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Multiple Choice Quiz

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Microscopy & Imaging

Multiple Choice Quiz



1

How does the scanning electron microscope differ from the transmission electron microscope? It:
A)uses electromagnetic lenses.
B)operates with a vacuum.
C)uses an electron beam.
D)maps images rather than optically projecting them.
E)produces monochrome images.
2

Resolution in a microscope is primarily determined by which lens?
A)Ocular
B)Condenser
C)Objective
D)Projector
E)Intermediate
3

Secondary electrons used in SEM have energies of:
A)less than 50 electron volts
B)50 – 150 electron volts
C)250 – 300 electron volts
D)greater than 500 electron volts
E)Varied energies in the above ranges depending on the atomic number of specimens.
4

Most noteworthy plant anatomist of the 20th century.
A)John Ray
B)Robert Hooke
C)Katherine Esau
D)Eduard Strasburger
E)Robert Brown
5

The primary limiting factor for resolution in a light microscope is the:
A)quality of the lenses.
B)numerical apertures.
C)index of refraction.
D)angle of illumination into the objective lens.
E)wavelength of light
6

Which instrument gives the most "3-dimensional-like" image (greatest Z-axis of focus)?
A)Compound light microscope
B)Transmission electron microscope
C)Scanning electron microscope
D)Atomic force microscope
E)A single-lens microscope
7

In its optical design, the transmission electron microscope is most like a(n):
A)scanning electron microscope.
B)atomic force microscope.
C)single-lens microscope.
D)confocal microscope.
E)compound light microscope (brightfield).
8

Which instrument is best used to view the structure of viruses? A___.
A)single-lens microscope
B)confocal microscope
C)scanning electron microscope
D)transmission electron microscope
E)compound light microscope (brightfield)
9

The primary advantage of a lens in a camera over a pinhole is:
A)greater light-gathering power.
B)a fixed focal length.
C)a small numerical aperture.
D)greater empty magnification.
E)lower cost.
10

The physical principles of microscope resolution was first determined by:
A)Ernst Abbe.
B)Koehler.
C)J. J. Thompson.
D)T. Everhardt.
E)Carl Zeiss.