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1 | |
List at least three medical uses for recombinant DNA technology. |
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2 | |
Why are sticky ends useful for gene cloning? |
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3 | |
What might be an advantage of using restriction enzymes that produce blunt ends? |
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4 | |
In addition to an origin of replication and a selectable marker, vectors often contain a screenable marker such as lacZ, which is disrupted by insertion of DNA. Why is this valuable? |
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5 | |
Once a hybrid vector has been transformed into a bacterial cell, what two processes contribute to production of many copies of the gene? |
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6 | |
What is the role of ligase in a cloning experiment? |
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7 | |
After cells were transformed with the first recombinant plasmid, they were plated on growth medium containing both tetracycline and kanamycin. What was the purpose of each antibiotic? |
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8 | |
If you wish to identify regulatory elements controlling a specific gene, would you start with genomic DNA or mRNA? |
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9 | |
When subcloning, how would you select which restriction enzymes to use? |
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10 | |
In addition to restriction mapping, how could you identify the location of restriction sites within a hybrid vector? |
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11 | |
How is PCR able to amplify only a specific region of DNA rather than the entire molecule? |
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12 | |
You have used transposon tagging to generate a strain of Drosophila that has poor memory, apparently because of insertion of the transposon in a specific gene. You tentatively name the gene "steel", as in "mind like a steel trap". How could you identify steel, using this strain of Drosophila? |
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13 | |
When using a DNA library, you must have a way to identify your DNA of interest. List three techniques you could use to do this. |
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14 | |
You have purified an enzyme that breaks large hydrocarbons down to basic building blocks. You would like to clone the gene for this enzyme and engineer it into bacteria that could then be used for everything from cleaning up large oil spills from tanker ships to removing oil spots from the family driveway. How would you go about this? |
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15 | |
Differentiate between Northern and Southern blotting. |
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16 | |
You have a genomic clone including the regulatory sequences of a gene. What techniques might you use to determine the location of transcription factor binding sites? |
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17 | |
What are examples of information that can be collected by DNA sequencing? |
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