| Study Outline (See related pages)
- Gene Cloning
- Cloning experiments usually involve two kinds of DNA molecules: chromosomal DNA and vector DNA
- Enzymes are used to cut DNA into pieces and join the pieces together
- Gene cloning involves the insertion of DNA fragments into vectors, which are then propagated within host cells
- In the first gene cloning experiment, Cohen, Chang, Boyer, and Helling inserted a kanamycinR gene into a plasmid vector
- cDNA can be made from mRNA via reverse transcriptase
- Restriction mapping is used to locate the restriction sites within a vector
- Polymerase chain reaction (PCR) can also be used to make many copies of DNA
- Detection of Genes and Gene Products
- A DNA library is a collection of many cloned fragments of DNA
- Blotting methods can be used to detect DNA sequences
- Gene products can also be detected by blotting methods
- Techniques can be used to detect the binding of proteins to DNA sequences
- Analysis and Alteration of DNA Sequences
- The dideoxy method of DNA sequencing is based on our knowledge of DNA replication
- In vitro site-directed mutagenesis is a technique to alter DNA sequences
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