First Experiment

1		The coding sequence for somatostatin came from
			A)	A cDNA clone
			B)	A genomic clone
			C)	The linkage of several oligonucleotides
			D)	None of the above
2		If somatostatin was not linked to?β-galactosidase,
			A)	It caused the bacterial cells to die.
			B)	It was rapidly degraded.
			C)	It was overproduced.
			D)	It formed a polymer that was difficult to purify.
3		When somatostatin was in the wrong orientation, we would expect that
			A)	It would be expressed with the reverse amino acid sequence.
			B)	It would be expressed, but not as a fusion protein.
			C)	It would not be expressed because it was not correctly linked to a promoter.
			D)	It would not be expressed because the promoter was not inducible with IPTG.
4		In this experiment, the purpose of adding IPTG was to
			A)	Turn on the lac promoter
			B)	Cause β-galactosidase to fuse with somatostatin
			C)	Cause?β-galactosidase to be separated from somatostatin
			D)	None of the above
5		The covalent bond between?β-galactosidase and somatostatin was broken by
			A)	IPTG
			B)	70% formic acid
			C)	CaCl2
			D)	Cyanogen bromide