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Short Answer Quiz
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1

What is the major benefit of functional genomics over more traditional methods of gene cloning and analysis?
2

You have a strain of mammalian cells in tissue culture, and an expression plasmid containing the gene that encodes a newly discovered transcription factor. How might you be able to identify all the genes that are differentially expressed in the presence of this transcription factor?
3

Studies of cancer progression have demonstrated that changes in gene expression signal a change in tumor characteristics even if the cells themselves look no different. Explain how microarray technology could use this information to improve diagnosis and treatment of cancer.
4

List factors that would be expected to influence the levels of protein activity in a cell.
5

Give two reasons that protein microarrays are more difficult to produce than DNA microarrays.
6

Give three types of information you could expect to obtain from a search of an annotated database of genetic information.
7

What elements of organization would allow you to predict that a particular DNA sequence includes a coding region for a protein?
8

How would you recognize highly similar sequences on a dot matrix?
9

How might an amino acid substitution disrupt the secondary structure of a protein?
10

The function of mass spectrometry is:





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