First Experiment

1		In this experiment, the purpose of column chromatography is to
			A)	Purify cDNA
			B)	Purify mRNA
			C)	Denature DNA
			D)	Purify proteins
2		The green cDNA would be made if a gene was expressed when
			A)	Glucose levels were relatively high.
			B)	Glucose levels were very low.
			C)	The TCA cycle is maximal.
			D)	Glycolysis is shut down.
3		The red cDNA would be made if a gene was expressed
			A)	During the peak phase of glycolysis
			B)	Any time after nine hours
			C)	When the TCA cycle is maximal
			D)	B and C
4		If the ratio of fluorescence (red divided by green) is greater than one, this indicates that a gene is
			A)	Induced after nine hours
			B)	Repressed after nine hours
			C)	Induced before nine hours
			D)	Repressed before nine hours
5		Assuming that the diauxic shift occurs around 17 hours, the genes that are repressed when the yeast cells shift to the TCA cycle are shown in the data of part
			A)	(a)
			B)	(b)
			C)	(c)
			D)	(d)