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Study Outline
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  1. Functional Genomics
    1. Expressed genes can be identified in a cDNA library
    2. A microarray can identify genes that are transcribed
    3. The coordinate regulation of many genes is revealed by a DNA microarray analysis
  2. Proteomics
    1. The proteome is much larger than the genome
    2. 2D gel electrophoresis is used to separate a mixture of cellular proteins
    3. Mass spectrometry is used to identify proteins
    4. Protein microarrays can be used to study protein expression and function
  3. Bioinformatics
    1. Sequence files are analyzed by computer programs
    2. The scientific community has collected sequence files and stored them in large computer databases
    3. Different computational strategies can identify functional genetic sequences
    4. Several computer-based approaches can identify structural genes within a nucleotide sequence
    5. Computer programs can identify homologous sequences
    6. A simple dot matrix can compare the degree of similarity between two sequences
    7. A database can be searched to identify homologous sequences
    8. Genetic sequences can be used to predict the structure of RNA and proteins





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