First Experiment

1		The purpose of adding calcium and phosphate ions was to
			A)	Break open the cells
			B)	Promote mutations in the normal mouse fibroblast cells
			C)	Enhance the growth of the cells
			D)	Promote the uptake of DNA into the cells
2		In this experiment, the identification of cancerous cells was based on
			A)	The growth of malignant foci
			B)	Their size and color
			C)	The amount of DNA per cell
			D)	Their growth rate in liquid cultures
3		The formation of malignant foci was caused by
			A)	The uptake of oncogenes
			B)	The uptake of mutant (loss of function) tumor suppressor genes
			C)	The uptake of normal tumor suppressor genes
			D)	The uptake of either oncogenes or mutant (loss of function) tumor suppressor genes
4		When DNA was isolated from normal mouse fibroblast cells and exposed to normal mouse fibroblast cells, malignant foci did not form because
			A)	The DNA was not taken up.
			B)	The DNA did not contain any oncogenes.
			C)	Uptake only occurs between different cell types.
			D)	None of the above
5		When the DNA came from two of the malignant cell lines, namely MB66 MCA ACL6 and MB66 MCA ACL13, no malignant foci were observed. One explanation for this result would be
			A)	These two strains carry mutant (loss of function) tumor suppressor genes.
			B)	These two strains have many different oncogenes.
			C)	These two strains have too much DNA.
			D)	None of the above