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| 1 | |
Identify the term that refers to a complete absence of viable microbes. |
| | A) | sterile |
| | B) | septic |
| | C) | aerobic |
| | D) | anaerobic |
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| 2 | |
Identify the major technique that refers to placement of bacteria into a medium such as broth, blood, or a fertilized egg in order to facilitate growth. |
| | A) | isolation |
| | B) | inspection |
| | C) | inoculation |
| | D) | identification |
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| 3 | |
Microbiologists take bacterial cells and plate them on a medium in order to grow a mound of cells adequate for study. What is the proper term for a mound of cells that develops after plating the bacteria on a suitable medium? |
| | A) | culture |
| | B) | growth |
| | C) | colony |
| | D) | clump |
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| 4 | |
Of the three categories of media classification, which of the following does not refer to the chemical composition of the medium? |
| | A) | general purpose |
| | B) | selective |
| | C) | differential |
| | D) | nonsynthetic |
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| 5 | |
Broths, milks, and infusions are all examples of |
| | A) | liquid media. |
| | B) | semi-liquid media. |
| | C) | solid media that will liquefy. |
| | D) | solid media that will not liquefy. |
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| 6 | |
If a microbiologist wanted to check for motility of bacteria, he would choose a medium that was |
| | A) | liquid. |
| | B) | semi-solid. |
| | C) | solid. |
| | D) | coagulated. |
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| 7 | |
What type of medium has a composition that is chemically defined? |
| | A) | synthetic |
| | B) | nonsynthetic |
| | C) | complex |
| | D) | infusion |
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| 8 | |
A _____________ medium promotes the growth of a specific microbe while inhibiting the growth of certain other microbes. |
| | A) | differential |
| | B) | selective |
| | C) | general purpose |
| | D) | nonsynthetic |
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| 9 | |
A microbiologist plated a bacterium on a specific medium. After incubation, she noted that the previously yellow medium was now hot pink and there were numerous bacterial colonies growing on the medium. What type of medium did the microbiologist use in this example? |
| | A) | selective |
| | B) | general purpose |
| | C) | nonsynthetic |
| | D) | differential |
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| 10 | |
Dyes are often added to growth media to aid in the identification of microbes. What could be the purpose of a dye in this case? |
| | A) | to make the plate prettier |
| | B) | to color key the type of plate being used |
| | C) | to act as a growth enhancer |
| | D) | to act as a pH indicator |
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| 11 | |
Blood agar is often used to differentiate between |
| | A) | salt sensitive and non-salt sensitive microbes. |
| | B) | bacteria that absorb oxygen and those that do not. |
| | C) | types of hemolysis the bacteria may produce. |
| | D) | all of the above. |
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| 12 | |
A transport medium is used to maintain and preserve specimens that have to be held for periods of time prior to clinical analysis. Transport media contain substances that prevent cell destruction such as |
| | A) | salts, buffers, and absorbants. |
| | B) | salts, acids, and bases. |
| | C) | toxins and buffers. |
| | D) | water and enzymes. |
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| 13 | |
Streak plates are most useful in |
| | A) | isolating individual cells. |
| | B) | allowing colony morphology to be observed. |
| | C) | separating different organisms. |
| | D) | all of the above. |
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| 14 | |
Most bacterial species grown in the laboratory are grown at temperatures between |
| | A) | 5° and 10° C. |
| | B) | 10° and 20° C. |
| | C) | 20° and 40° C. |
| | D) | 40° and 90° C. |
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| 15 | |
How do microbiologists identify microorganisms? |
| | A) | macroscopic appearance |
| | B) | microscopic morphology |
| | C) | biochemical reactions |
| | D) | all of the above |
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| 16 | |
The bending of a light ray as it passes through a medium such as a lens is known as ___. |
| | A) | revolving |
| | B) | refraction |
| | C) | magnification |
| | D) | resolution |
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| 17 | |
A student views a specimen with a light microscope. The ocular lens used is a 4x and the objective lens is 40x. What is the total magnification of the specimen viewed? |
| | A) | 36x |
| | B) | 40x |
| | C) | 44x |
| | D) | 160x |
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| 18 | |
The most widely used light microscope transmits light through a live specimen to produce an image where the specimen is darker than its surroundings. What type of microscope is this? |
| | A) | bright-field |
| | B) | dark-field |
| | C) | differential interference |
| | D) | electron |
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| 19 | |
What type of microscope is specially modified with an ultraviolet radiation source and a filter? |
| | A) | bright-field |
| | B) | dark-field |
| | C) | phase-contrast |
| | D) | fluorescent |
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| 20 | |
An electron microscope has a higher resolution, or ability to see small things, than a light microscope. This is because electrons |
| | A) | pass through objects more easily. |
| | B) | have a shorter wavelength. |
| | C) | outline specimens more readily. |
| | D) | immobilize the object they are magnifying. |
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| 21 | |
In a ________________ stain, the background is stained and the organism appears clear against a dark background. |
| | A) | positive |
| | B) | negative |
| | C) | Gram |
| | D) | differential |
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| 22 | |
A stain technique that uses only one dye is termed a __________ stain. |
| | A) | positive |
| | B) | differential |
| | C) | simple |
| | D) | Gram |
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| 23 | |
A Gram stain uses a primary dye to stain Gram-positive organisms and a counterstain to dye Gram-negative organisms. What color will a Gram-negative specimen be? |
| | A) | clear |
| | B) | purple |
| | C) | green |
| | D) | red |
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| 24 | |
A microbiologist is attempting to check for the presence of Mycobacterium tuberculosis in a sputum sample. This particular bacterium, if present, would have a particularly impervious outer wall. What type staining technique might be used to check for this bacterium? |
| | A) | Gram stain |
| | B) | simple stain |
| | C) | negative stain |
| | D) | acid-fast stain |
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| 25 | |
A patient has a leg wound that is seeping and emitting a strong odor. The doctor suspects Clostridium bacteria may be present. What type of staining procedure might be used? |
| | A) | simple stain |
| | B) | spore stain |
| | C) | negative stain |
| | D) | acid-fast stain |
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| 26 | |
What is the first step in the Five I's? |
| | A) | inspection |
| | B) | identification |
| | C) | incubation |
| | D) | inoculation |
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| 27 | |
The purpose of an incubator is to recreate the proper growth conditions of a bacterial sample in order to promote bacterial growth. |
| | A) | True |
| | B) | False |
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| 28 | |
Media can be either selective or differential, but they cannot be both. |
| | A) | True |
| | B) | False |
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| 29 | |
The hanging drop preparation is useful when observing biochemical reactions |
| | A) | True |
| | B) | False |
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| 30 | |
Sabouraud's agar is slightly acidic to aid in the growth of fungi. We would classify this medium as differential. |
| | A) | True |
| | B) | False |
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| 31 | |
Reducing media is used primarily to grow aerobic bacteria. |
| | A) | True |
| | B) | False |
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| 32 | |
An anexic culture contains more than one species of bacteria. |
| | A) | True |
| | B) | False |
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| 33 | |
Capsules of pathogenic bacteria can be readily viewed using the Gram stain. |
| | A) | True |
| | B) | False |
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