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Short Answer Quiz
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1

Describe a genome.
2

What are the four important roles of DNA as described by your text?
3

You are studying a new, virulent disease outbreak. Finally, the infectious agent is demonstrated to be a nonenveloped virus with a simple shell of identical protein subunits. How would you expect the chromosome of this virus to compare to those listed in table 10.1?
4

Describe the levels of compaction of the bacterial chromosome.
5

What is the difference between positive and negative supercoiling?
6

How is an understanding of the function of bacterial topoisomerases useful for human medicine?
7

What eukaryotic subcellular structure is functionally similar to the bacterial nucleoid?
8

What are the satellite peaks of DNA?
9

Why is the slope of the highly repetitive DNA much more shallow than that of the single copy DNA in a Cot curve such as the one shown in Fig 10.13?
10

Define chromatin remodeling.
11

What is the approximate size and composition of one nucleosome?
12

What might explain the fact that histone proteins have a strong positive charge due to charged lysine and arginine amino acids?
13

What is the effect of low salt concentrations on DNA packaging?
14

What is the currently accepted model for the structure of the 30nm fiber?
15

What is the function of an MAR?
16

Describe facultative heterochromatin.
17

Why must M phase be a relatively short portion of the cell cycle?
18

What is the role of cohesin in cell division?
19

Compare condensation of bacterial and eukaryotic chromosomes.





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