First Experiment

1		The purpose of the addition of phenol was to
			A)	Break the DNA into small fragments
			B)	Separate the DNA and proteins
			C)	Denature the double-stranded DNA
			D)	None of the above
2		When subjected to gradient centrifugation, repetitive DNA may sediment at a different density compared to other chromosome DNA because
			A)	It is broken into longer DNA fragments.
			B)	It is the most abundant form of DNA.
			C)	It is the least abundant form of DNA.
			D)	Its base composition may be different from the other chromosomal DNA.
3		The purpose of separating the gradient into fractions is to
			A)	Separate molecules with different densities
			B)	Identify fractions that don't contain DNA
			C)	Denature the DNA
			D)	Break the DNA into small fragments
4		The two small peaks with densities of 1.672 and 1.688 represent
			A)	Smaller DNA fragments
			B)	Larger DNA fragments
			C)	Repetitive DNA fragments
			D)	Nonrepetitive DNA fragments
5		If Drosophila contained a repetitive DNA sequence that was 100% GC, the data would have shown another peak at what location in the DNA fractions?
			A)	In an earlier fraction (i.e., a higher density than 1.701).
			B)	In an earlier fraction (i.e., a lower density than 1.701)
			C)	In a later fraction (i.e., a higher density than 1.701)
			D)	In a later fraction (i.e., a lower density than 1.701)

Second Experiment

6		If the linker for eukaryotic DNA was 450 bp instead of 50 bp, how would that have affected the results?
			A)	The results would be the same.
			B)	The DNA fragments would be in multiples of 400 bp.
			C)	The DNA fragments would be in multiples of 600 bp.
			D)	The DNA fragments would be in multiples of 800 bp.
7		A low concentration of DNase I is expected to
			A)	Always cut the linker region
			B)	Occasionally cut the linker region
			C)	Always cut the DNA wrapped around the histones
			D)	None of the above
8		In this experiment, the purpose of gel electrophoresis is to
			A)	Denature the DNA
			B)	Identify the histone proteins
			C)	Remove the histone proteins
			D)	Identify the sizes of the DNA fragments
9		If this experiment was conducted on bacterial DNA, we would expect
			A)	The same results
			B)	The sizes of the DNA fragments would be random.
			C)	The fragments would be in multiples of 1000bp.
			D)	The fragments would be in multiples of 400bp.
10		If ethidium bromide was accidentally omitted from the experiment, how would the results be affected?
			A)	The DNA fragments would migrate faster.
			B)	The DNA fragments would migrate slower.
			C)	It would not be possible to see the DNA fragments.
			D)	DNase I would not be able to digest the DNA.