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Short Answer Quiz
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1

In genetics, what process does the term "transcription" describe?
2

What is the difference between the closed promoter complex and the open promoter complex?
3

During which stages of transcription does the open promoter complex exist?
4

What conclusion might you draw from the comparison of Pribnow (-10) sequences in fig 12.4?
5

Describe the functions of the various components of the RNA polymerase holoenzyme in E. coli.
6

Approximately how long would you expect RNA polymerase to take to transcribe the coding sequence of a protein that contains 500 amino acids?
7

What is the key similarity and difference between ρ dependent and ρ independent transcription termination?
8

What eukaryotic promoter element is most similar to the Pribnow box found in prokaryotes?
9

What is the functional difference between eukaryotic RNA polymerases?
10

What general transcription factors are required to form the open complex in eukaryotes?
11

List at least three factors which would be expected to regulate gene expression in eukaryotes.
12

Which types of modification of RNAs are common for tRNA molecules?
13

Describe a eukaryotic transcript that might be considered similar to a polycistronic transcript in prokaryotes.
14

If you were searching for the sequence of a gene, would you rather use a genomic or a cDNA library?
15

List the components of a spliceosome.
16

What are potential functions of alternative splicing?





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