| Study Outline (See related pages)
- Overview of Transcription
- Gene expression requires base sequences that perform different functional roles
- The three stages of transcription are initiation, elongation, and termination
- RNA transcripts have different functions
- Transcription in bacteria
- A promoter is a short sequence of DNA that is necessary to initiate transcription
- Bacterial transcription is initiated when RNA polymerase holoenzyme binds at a promoter sequence
- The RNA transcript is synthesized during the elongation stage
- Transcription is terminated by either an RNA-binding protein or an intrinsic terminator
- Transcription in Eukaryotes
- Eukaryotes have multiple RNA polymerases that are structurally similar to the bacterial enzyme
- Eukaryotic structural genes have a core promoter and regulatory elements
- Transcription of eukaryotic structural genes is initiated when RNA polymerase II and general transcription factors bind to a promoter sequence
- Chromatin structure plays a key role in gene transcription
- RNA Modification
- Some large RNA transcripts are processed to smaller functional transcripts by enzymatic cleavage
- Introns were experimentally identified via microscopy
- Three different splicing mechanisms can remove introns
- The ends of eukaryotic pre-mRNAs have a 5' cap and a 3' tail
- Pre-mRNA splicing occurs by the action of a spliceosome
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