First Experiment

1		The RNA that causes an R loop to form binds to
			A)	The template strand (i.e., noncoding strand) of the DNA
			B)	The coding strand of the DNA
			C)	Both strands of DNA
			D)	Neither strand of DNA
2		In this experiment, an intron forms a double stranded region because
			A)	The DNA was not properly denatured.
			B)	The intron is very long.
			C)	The intron is complementary to regions that were spliced out of the RNA.
			D)	The intron is AT rich.
3		If a gene contained three introns, in this experiment it would form
			A)	3 R loops and 3 double-stranded intron regions
			B)	4 R loops and 3 double-stranded intron regions
			C)	3 R loops and 2 double-stranded intron regions
			D)	4 R loops and 2 double-stranded intron regions
4		What is the purpose of the addition of formamide and the incubation at 52oC?
			A)	To denature the RNA
			B)	To denature the DNA and allow RNA to bind
			C)	To prevent introns from forming
			D)	To promote introns to form
5		If we started with cDNA for the β-globin gene instead of a chromosomal DNA clone, how the results be different?
			A)	The results would be the same.
			B)	More introns would be observed.
			C)	More R loops would be observed.
			D)	No introns would be observed.