Choose the best answer.
|
16 | | The reduced numbers of progeny with the pattern of (5,000bp, 4,300bp, 2,300bp, and 2,000bp bands) or (5,000bp, 4,300bp, and 3,350bp bands) indicates a selective disadvantage for these genotypes. |
| | A) | True |
| | B) | False |
|
|
17 | | What was the arrangement of the markers in the original parental strains? |
| | A) | A: 5,000bp, B: 4,300bp and A: 3,350bp, B: 2,300/2000bp |
| | B) | A: 5,000bp, B: 2,300/2,000bp and A: 3,350bp, B: 4,300bp |
| | C) | A: 3,350bp, B: 4,300bp and A: 5,000bp, B: 4,300bp |
| | D) | A: 3,350bp, B: 2,300/2000bp and A: 3,350bp, B: 2,300/2000bp |
|
|
18 | | What is the distance between these two markers? |
| | A) | 0.167mu. |
| | B) | 0.20mu. |
| | C) | 16.7mu. |
| | D) | 20mu. |
| | E) | None of these. |
|
|
19 | | Which of the following would be a reasonable use of an RFLP map? |
| | A) | Identification of the exact location of an unknown gene along the chromosome. |
| | B) | Description of the size of DNA fragment required to clone the region containing an unknown gene. |
| | C) | Identification of the region in which an unknown gene is located. |
| | D) | All of these. |
| | E) | None of these. |
|
|
20 | | Which of these genetic markers is most likely to be highly polymorphic (have many different alleles)? |
| | A) | An RFLP. |
| | B) | A microsatellite. |
| | C) | An SNP. |
| | D) | All of these are equally polymorphic. |
| | E) | None of these are likely to be polymorphic. |
|
|
21 | | Why might use of microsatellites in genetic mapping studies be an advantage over RFLPs? |
| | A) | Microsatellites are easier to detect. |
| | B) | Microsatellites are more abundant than RFLPs. |
| | C) | Microsatellites have more potential alleles than RFLPs. |
| | D) | All of these. |
| | E) | None of these. |
|
|
22 | | Isolation of individual chromosomes can be used to map genes. |
| | A) | True |
| | B) | False |
|
|
23 | | How are individual chromosomes identified in chromosome sorting techniques? |
| | A) | Relative level of fluorescence when stained with a dye mixture. |
| | B) | Level of charge relative to size. |
| | C) | Size of the molecule. |
| | D) | All of these. |
| | E) | None of these. |
|
|
24 | | Which of these describes a contig? |
| | A) | A complete genomic library including overlapping clones. |
| | B) | A complete mRNA library. |
| | C) | A chromosome-specific library of overlapping clones. |
| | D) | An ordered genomic library. |
|
|
25 | | What vector would be best suited for creating a contig of bovine (cattle) chromosome 10? |
| | A) | λ? |
| | B) | A plasmid. |
| | C) | A YAc. |
| | D) | Any of these. |
| | E) | None of these. |
|
|
26 | | Which of the following would not be a critical characteristic of a YAC vector? |
| | A) | Telomeric sequences. |
| | B) | A gene encoding a required structural protein. |
| | C) | An origin of replication. |
| | D) | A centromere. |
| | E) | All of these are critical characteristics of a YAC. |
|
|
27 | | After cytogenetic and linkage mapping, you have identified a relatively small area of a chromosome where your gene of interest is likely to reside. You decide to attempt to use a YAC contig to map the gene by positional cloning. How might you identify the clone that may contain the gene? |
| | A) | The clone will also contain the nearest marker mapped by linkage analysis. |
| | B) | The DNA sequence of the clone will contain an ORF (open reading frame). |
| | C) | Cytological hybridization of the clone produces a different pattern than that of the nearest mapped marker. |
| | D) | All of these. |
| | E) | None of these. |
|
|
28 | | Which of these is a reasonable use for the contigs and clones generated in the process of obtaining a physical map of the human genome? |
| | A) | Correlation of physical and linkage map distances to determine recombination frequency. |
| | B) | Identification of all the known alleles of a single disease-causing gene. |
| | C) | Insertion into cells affected with genetic disease in the process of gene therapy. |
| | D) | All of these. |
| | E) | None of these. |
|
|
29 | | One of the goals of the Human Genome Project is to address ethical issues arising from use of the information gathered. |
| | A) | True |
| | B) | False |
|
|
30 | | Sequencing of genomes other than humans is potentially valuable because: |
| | A) | Disorders of cellular function can be studied in relatively simple model systems. |
| | B) | Comparison of gene sequences between different species can allow prediction of disease-causing mutations. |
| | C) | Genes may have a similar function in other species, giving us a place to start with functional analysis of similar genes. |
| | D) | None of these. |
|