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Tweaking the Experiment
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First Experiment



1

The reason why a mutant gene is likely to be associated with a particular RFLP is because
A)The mutation in the gene changes a restriction site.
B)The mutation in the gene is a deletion.
C)The original mutation occurred in a single founder, and everyone has RFLPs that are within or closely linked to particular genes.
D)None of the above
2

The purpose of adding HpaI was to
A)Denature the DNA
B)Digest the DNA into short fragments
C)Link the β-globin gene to an RFLP
D)Separate the β-globin gene from an RFLP
3

In this experiment, the Southern blotting procedure detected
A)A DNA fragment of the β-globin gene
B)The mutation within the β-globin gene
C)Only the RFLP, but not the β-globin gene
D)None of the above
4

If an individual was 13.0/7.0, it would be most likely for that individual to be
A)Homozygous for the sickle cell allele
B)Homozygous for the normal allele
C)Heterozygous for the two alleles
D)None of the above
5

It is occasionally possible for an individual to carry the sickle-cell allele and yet not have the 13.0 RFLP. If the founder hypothesis is correct, what is the most likely explanation?
A)A second mutation created a new HpaI site.
B)A second mutation eliminated an HpaI site.
C)A second mutation caused a deletion.
D)In a heterozygous individual, a crossover occurred between the original mutation (in a 13.0 individual) and an HpaI site in a 7.0 or 7.6 individual.







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