Biology (Mader), 10th Edition

Chapter 14: Biotechnology and Genomics

Learning Outcomes

After studying this chapter, you should be able to accomplish the following outcomes.

DNA Cloning

1. Define what is meant by recombinant DNA (rDNA), and list the most common laboratory techniques used to generate it.
2. Explain how the polymerase chain reaction can be used to amplify DNA segments, and how it may be used to analyze DNA.

Biotechnology Products

3. Describe how transgenic bacteria, plants, and animals may be used to generate products useful to humans.

4. Contrast in vivo and ex vivo gene therapy techniques.
5. List some disorders in humans that gene therapy has been used to treat.

6. Describe what has been learned from the Human Genome Project.
7. Describe the arrangement of genes on human chromosomes.
8. List the three types of intergenic sequences and describe the properties of each.
9. Explain why the definition of gene is being reconsidered to include DNA sequences that do not encode proteins.