Biology, Eighth Edition (Raven)

Chapter 17: Biotechnology

Learning Outcomes

Chapter 17
  • Understand the importance of plasmids and viruses to genetic engineering.
  • Know the natural function of restriction endonucleases and how a normal bacterial cell protects its DNA from their activity.
  • Understand how “sticky ends” are formed and their importance to gene technology.
  • Describe how a chimeric genome is constructed.
  • Explain the four steps of genetic engineering experiments.
  • Distinguish between the techniques of selection and screening of clones.
  • Explain how to screen for clones that contain a desired gene fragment.
  • Understand the value of and the processes involved with the polymerase chain reaction (PCR).
  • Describe techniques used to characterize DNA.
  • Discuss the different applications of gene technology.
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