Biology, Eighth Edition (Raven)

Chapter 17: Biotechnology

Polymerase Chain Reaction

View the animation below, then complete the quiz to test your knowledge of the concept.



1.

PCR requires all of the following EXCEPT
A)primers.
B)DNA ligase.
C)DNA polymerase.
D)DNA of interest.
E)deoxyrobinucleotides.
2.

If you start with one double-stranded DNA molecule and you perform SIX cycles of PCR, how many double-stranded copies of the DNA will you have?
A)6
B)8
C)16
D)32
E)64
3.

The most likely source of the Taq polymerase used in PCR is a bacterium that lives in
A)soil.
B)arctic ice.
C)hot vents.
D)humans.
E)plants.
4.

PCR can be used to amplify DNA from any source.
A)True
B)False
5.

During the PCR, the hydrogen bonds of the double-stranded DNA molecules are broken by the enzyme helicase.
A)True
B)False
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